ACCURACY AND PRECISION OF NORMAL LEVEL CONTROL SERUM USING SEMI-AUTOMATIC AND FULLY AUTOMATIC ANALYZER METHODS FOR UREA AND URIC ACID PARAMETERS

Abstract

Background: Clinical laboratories need to be conducted with quality to support efforts to improve quality. Method validation in the laboratory is a quality control process aimed at ensuring that test results have good accuracy and precision so they can be trusted. Testing urea and uric acid levels is an important part of diagnosing kidney function disorders. Advances in clinical laboratory technology have impacted analytical method instruments, evolving from semi automatic to fully automatic. Object: The purpose of this study is to determine the accuracy and precision of normal level control serum for urea and uric acid parameters using semi automatic and fully automatic analyzers. Method: The type of research used in this study is comparative analytic with a cross-sectional approach. This study used commercial control serum at the normal level tested with both types of analyzers, semi automatic and fully automatic, then analyzed for inaccuracy and imprecision values. Result: The % bias value of the semi-automatic method on urea levels is 5.48% and uric acid levels is 4.73%. The % bias value of the fully automatic method on urea levels is 4.27% and uric acid levels is 3.43%. The CV% value of the semi-automatic method on urea levels is 6.30% and uric acid levels is 5.26%. The CV% value of the fully automatic analyzer method on urea levels is 4.86% and uric acid levels is 4.61%. Conclusion: Overall, both methods for testing urea and uric acid have good accuracy and precision, but the fully automatic method has better accuracy and precision