GENETIC DETECTION OF AEDES AEGYPTI MOSQUITO RESISTANCE TO PYRETHROID INSECTICIDE

Abstract

Background: Aedes aegypti mosquitoes are the main vectors of dengue fever, which is endemic in tropical and subtropical countries, including Indonesia. Continuous vector control efforts have been carried out to reduce dengue cases. As a preparedness measure, a molecular approach such as VGSC gene detection is needed to identify the resistance of Aedes aegypti mosquitoes to pyrethroid insecticides.  Object: This study aims to determine the results of VGSC gene detection as an indication of resistance in Aedes aegypti mosquitoes exposed to fenvalerate insecticide using the Real-Time PCR method. Method: This quantitative descriptive study used observational data analysis and the CDC Bottle Assay method, consisting of four test bottles and one control bottle. After the resistance test, mosquito samples were processed into suspensions for DNA extraction, followed by testing for DNA purity and concentration. VGSC gene detection was performed using the Real-Time PCR method, with results expressed as cycle threshold (CT) values. Results: The findings showed that out of four mosquito samples tested, two samples (R1 and R4) were positive for the VGSC gene, with CT values of 1.67 and 1.63, respectively, while the other two samples (R2 and R3) showed N/A results, indicating no VGSC gene detected. Conclusion: Needs follow-up and innovation from health workers and cross-sectors to increase the use of postpartum family planning. Based on these results, it can be concluded that 50% of Aedes aegypti mosquito samples showed the presence of the VGSC gene, while the remaining 50% did not.