VARIATION OF ANTICOAGULANT AND STORAGE TIME ON PRP QUALITY

Abstract

ABSTRACT .Different procedures of PRP preparation can affect the platelet count in PRP. Platelet
count is one of the factors that determines the quality of PRP. This study aimed to describe the
number of platelets based on variations in anticoagulants and storage time in PRP. This study was
an experimental study with a pre-post test only control group design. PRP was processed from whole
blood from 9 respondents. Whole blood is collected in a tube containing Sodium Citrate, EDTA, and
ACD-A anticoagulants. Each tube will be processed into PRP through 2 stages of centrifugation.
The number of platelets in PRP was counted immediately (0 hour) and counted after 24 hours of
storage. The results showed that PRP processed using EDTA had the highest platelet count compared
to Sodium Citrate and ACD-A at 0 hour of storage, but decreased at 24 hours of storage. The number
of PRP platelets in ACD-A and Sodium Citrate tubes increased after 24 hours of storage, but the best
platelet counts were found in ACD-A tubes. The use of anticoagulants in the manufacture of PRP and
the storage time of PRP resulted in varying PRP platelet counts. This study must be carried out further
in order to determine the concentration of growth factors based on the use of anticoagulants and the
storage time of PRP.